Wastewater-based epidemiological surveillance to monitor the prevalence of SARS-CoV-2 in developing countries with onsite sanitation facilitiesArticle Published on 2022-10-152022-11-15 Journal: Environmental pollution (Barking, Essex : 1987) [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] absence approach collected Complete Contagion correlation country COVID-19 COVID-19 pandemic Critical detect Developing developing countries disease drain epidemiological Epidemiology facilitate genetic component genetic material help highest identification investigated Isolation MONITOR N genes National National surveillance program Onsite sanitation ORF1ab outbreak pandemic positive Prevalence reported RT-qPCR Rural Hotspot based sampling sampling SARS-CoV-2 SARS-CoV-2 monitoring SARS-CoV-2 RNA service Surveillance urban Urban Drain based sampling viral copy number Wastewater-based epidemiology (WBE). were used [DOI] 10.1016/j.envpol.2022.119679 PMC 바로가기
Elucidation of correlation between SARS-CoV-2 RdRp and N gene cycle threshold (Ct) by RT-PCR with age and gender연령 및 성별에 따른 RT-PCR에 의한 SARS-CoV-2 RdRp와 N 유전자 주기 역치(Ct) 간의 상관관계 규명Article Published on 2022-08-012022-09-11 Journal: Clinica chimica acta; international journal of cli [Category] COVID19(2023년), SARS, 치료법, [키워드] Abu acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 age age and gender Analysis association caused children conducted coronavirus coronavirus disease Coronavirus disease 2019 correlation COVID-19 COVID-19 patients COVID19 Ct value Ct values cycle cycle threshold Diseases dynamic Factor female females Gender global public health global public health emergency implication in viral individual longitudinal longitudinal analysis male mutated N gene N genes no statistically significant difference outcome P-value prevalent ranged RdRP RdRp gene risk rRT-PCR rRT-PCR. RT-PCR SARS-CoV-2 SARS-CoV-2 RdRp second wave significantly higher statistically significant difference strain study period target threshold Viral load viral loads [DOI] 10.1016/j.cca.2022.06.008 PMC 바로가기 [Article Type] Article
Characterization of SARS-CoV-2 Spike mutations important for infection of mice and escape from human immune sera쥐 감염 및 인간 면역 혈청 탈출에 중요한 SARS-CoV-2 스파이크 돌연변이의 특성화Article Published on 2022-07-072022-09-11 Journal: Nature Communications [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단, 치료기술, [키워드] (alpha ACE-2 Alpha angiotensin angiotensin converting enzyme B.1.1.529 B.1.1.7 B.1.351 Beta cause characterization convalescent disease Efficiency enzyme Gamma genetic background H655Y immune infect Infection insertion Lineage lineages lung mice Mild Mild disease morbidity murine Mutation N genes N501Y neutralization not affect obese omicron Omicron variants P.1 post vaccination receptor Receptor binding domain S gene S protein SARS-CoV-2 SARS-CoV-2 isolate SARS-CoV-2 isolates SARS-CoV-2 Spike mutation SARS-CoV-2 variant SARS-CoV-2 variants sera Transmissibility USA-WA1/2020 variants virus viruses VOCs wild type [DOI] 10.1038/s41467-022-30763-0 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Delta Variant N Gene Mutations Reduce Sensitivity to the TaqPath COVID-19 Multiplex Molecular Diagnostic AssaySARS-CoV-2 Delta 변이체 N 유전자 돌연변이로 TaqPath COVID-19 다중 분자 진단 분석에 대한 민감도 감소Article Published on 2022-06-162022-09-11 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단, 치료기술, 치료제, [키워드] assays CDC circulating cloned Control COVID-19 Delta delta variant diagnostic displaying genomic sequencing Guidance highlight investigated Lineage MONITOR Mutation N gene N gene dropout N genes PCR PCR assays Plasmid plasmids polymorphism qRT-PCR qRT-PCR. reduced saliva specimens SARS-CoV-2 SARS-CoV-2 genome sequences SARS-CoV-2 qRT-PCR SARS-CoV-2 virus sensitivity sequence TaqPath the SARS-CoV-2 the SARS-CoV-2 genome the SARS-CoV-2 virus [DOI] 10.3390/v14061316 PMC 바로가기 [Article Type] Article
Development and validation of a PCR-free nucleic acid testing method for RNA viruses based on linear molecular beacon probes선형 분자 비콘 프로브를 기반으로 하는 RNA 바이러스에 대한 PCR-free 핵산 검사 방법 개발 및 검증Article Published on 2022-06-112022-09-11 Journal: Journal of Nanobiotechnology [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification analyses applicability bind classical complementary Concentration confocal microscopy coronavirus CSFV Detection limit development diagnostic distribution domain double-stranded FAM Fever fluorophore host cells human diseases linear molecular Molecular beacon probes N genes nucleic acid nucleic acid extraction nucleic acid testing nucleic acids Pandemics PCR-free platform POC point-of-care probe qRT-PCR Quantitative RNA RNA virus RNA virus RNA viruses SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 N gene SARS-CoV-2. severe acute respiratory syndrome Coronavirus supported tissues viral nucleic acid viral nucleic acids virus virus were used [DOI] 10.1186/s12951-022-01470-1 PMC 바로가기 [Article Type] Article
Molecular Analysis of SARS-CoV-2 Lineages in Armenia아르메니아에서 SARS-CoV-2 계통의 분자 분석Article Published on 2022-05-172022-09-11 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] (Alpha) affected Alpha analysis Analysis Armenia caused circulation clade coronavirus COVID-19 Delta Epidemiology epitope Epitopes functional Genome genomic HLA Illumina Illumina sequencing information Lineage loci molecular Molecular epidemiology Mutation N gene N genes Nanopore nanopore sequencing omicron ORF1 ORF1ab PCR test PCR tests performed positive Protective provide Research RT-PCR SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sequence sequences Sequencing short-read sequencing signal specific clade specific clades Surveillance Transmission utility variants Viral viral evolution virus whole-genome sequencing Whole-genome sequencing. [DOI] 10.3390/v14051074 PMC 바로가기 [Article Type] Article
Ex vivo and in vivo suppression of SARS-CoV-2 with combinatorial AAV/RNAi expression vectors조합 AAV/RNAi 발현 벡터를 사용한 SARS-CoV-2의 생체외 및 생체내 억제Article Published on 2022-05-042022-09-11 Journal: Molecular therapy : the journal of the American So [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] AAV acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition adeno-associated virus Antiviral Antiviral agents antiviral RNAi Cell clinically cocktail complement coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 disease symptom effective escape mutant escape mutants experiment expression vector flexible Genetic global effort Gut hACE2 in vivo infected cell infected cells intestinal cells intranasal mice mutational escape. N gene N genes organoids pandemic prevented RdRP RNA RNAi SARS virus SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RdRp SARS-CoV-2 strains severe acute respiratory syndrome Coronavirus suppressed the SARS-CoV-2 therapeutic translation triggers Vaccine vector vectors viral adaptation Viral load viral loads virus wild-type [DOI] 10.1016/j.ymthe.2022.01.024 PMC 바로가기 [Article Type] Article
A localized small-scale external quality assessment (EQA) for PCR testing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the molecular laboratories분자 실험실에서 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2)의 PCR 검사를 위한 국지적 소규모 외부 품질 평가(EQA)Article Published on 2022-03-012022-09-11 Journal: Journal of virological methods [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome analyzed coronavirus Ct values cycle threshold E gene EQA Follow-up gene target Laboratory molecular N gene N genes N or E ORF1 ORF1ab participant pathogen Pathogens PCR PCR testing positive positive result positive sample Qualitative reported robust SARS-CoV-2 SARS-CoV-2 PCR testing SARS-CoV-2 RNA SARS-CoV-2 testing SARS-CoV-2. submitted Target genes the mean Variability [DOI] 10.1016/j.jviromet.2021.114441 PMC 바로가기 [Article Type] Article
Multiplex real-time RT-PCR method for the diagnosis of SARS-CoV-2 by targeting viral N , RdRP and human RP genesArticle Published on 2022-02-182022-10-04 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome applied Biological techniques can be used caused Corona coronavirus COVID-19 detect diagnosis of SARS-CoV-2 diagnostic disease gold standard hospitals human gene Laboratory limit of detection Molecular medicine multiplex Mutation N genes pandemic polymerase chain positive positive sample protocol real-time RT-PCR relative sensitivity Research SARS-CoV-2 SARS-CoV-2 RT-PCR sensitivity Spread tested variants viral gene viral genome virus [DOI] 10.1038/s41598-022-06977-z PMC 바로가기 [Article Type] Article
Field evaluation of seven SARS-COV-2 antigen assays7가지 SARS-COV-2 항원 분석의 현장 평가Article Published on 2022-02-012022-09-11 Journal: Journal of infection and public health [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Antigen asymptomatic patient asymptomatic patients Compliance coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cycle threshold detection rate diagnostic diagnostic test enrolled evaluate evaluated Evaluation false false-negative rate false-negative rates field median value median values Molecular assay molecular assays N genes PCR predicted ranged Rapid Rapid diagnostic test RdRP RDT RDTs RT-PCR SARS-CoV-2 SARS-CoV-2. Seven specimen specimens swab specimen target gene viral burden were used [DOI] 10.1016/j.jiph.2021.12.012 PMC 바로가기 [Article Type] Article